12/2/19-7 trout fry were removed. 0.5 grams of size 0 food was given to the trout. I used a pipette to remove fish waste. I noticed that one of the fry that was removed had just the head and a small amount of spine. It appeared that a fish had eaten its tail...(see picture below)
Trout count

• 194 living trout
• 72 dead trout​

Since there was an early dismissal today due to snow, and because I wanted to take precautionary measures in case the power goes out at school, a student helped me change out 3 gallons of trout tank water with clean water. 5 mL of Nitrifying bacteria was also added.
12/3/19- 3 trout fry were removed. 0.5 grams of size 0 food were fed to the trout. I tested the ammonia levels and it was a solid 0.5 ppm.
Trout count

• 191 living trout
• 75 dead trout​

12/4/19- 3 trout fry was removed.  I used a pipette to remove fish waste. You can see a picture of what that looked like in a beaker after I removed it from the tank. 0.3 grams of size 0 food were fed to the trout.
Trout count

• 188 living trout
• 78 dead trout​

12/5/19-
12/6/19-

11/25/19- 1 dead fry was removed (see picture below). Fish waste was removed from the basket. 0.5 grams of size 0 food was fed to the trout. 
WATER QUALITY UPDATE

• pH = 7.2
• Ammonia = 0.5 ppm
• Nitrates = 5.0 ppm

Trout count

• 210 living trout
• 56 dead trout

11/26/19- 1 dead fry was removed. A student used a pipette to remove fish waste. 0.5 grams of size 0 food was fed to the trout. I removed the cover to the filter intake and washed off the waste that had collected on it. I'm hoping this will help improve the water quality since it appears some of the fish food was getting sucked into it.
11/27/19- 4 dead fry were removed. A student used a pipette to remove fish waste. 0.5 grams of size 0 food was fed to the trout.
Trout count

• 205 living trout
• 61 dead trout​

11/27/19- 4 dead fry were removed. I used a pipette to remove some fish waste. A pinch of size 0 food was fed.
Trout count

• 201 living trout
• 65 dead trout​

Below is a picture of the fry that was removed on 11/25/19. You can see that it did not completely absorb its yolk sac, as there is a bit of an air bubble that can be viewed on the picture to the left. This was also a fry that was not eating, so its cause of death would be starvation.

11/18/19- I fed 0.3 grams of size 0 food to the trout.
11/19/19- I fed 0.3 grams of size 0 food to the trout. I used a pipette to remove some of the fish waste that gathered at the bottom of the basket. This is how you know the fish are eating and digesting!
11/20/19- I fed 0.5 grams of size 0 food to the trout. I used a pipette to remove some of the fish waste that gathered at the bottom of the basket. 
11/21/19- N/A
11/22/19- I fed 0.5 grams of size 0 food to the trout. I used a pipette to remove some of the fish waste that gathered at the bottom of the basket. 
WATER QUALITY UPDATE

• pH= 7.2
• Ammonia = 0.25 ppm
• Nitrites = 0 ppm
• ​Nitrates = 5 ppm

Now that the trout have absorbed and eaten the food in their yolk-sacs, they are no longer "sac-fry," but are just called "fry." Once they begin to develop lines on their bodies and approach 1 inch in length, they will be called fingerlings.

11/11/19- Some of the trout began swimming to the surface! In a few days, when more trout are swimming to the surface, we will begin feeding them!
11/12/19- No change.
11/13/19-  A dash of size 0 food was put into the water, but I didn't see any trout eat it.
11/14/19- 10 mL of Special Blend was added to the tank as maintenance. A dash of size 0 food was put into the water, and it looks like a few trout are beginning to eat. Check out the video below!
11/15/19- A dash of size 0 food was put into the water.
WATER QUALITY UPDATE

• pH=7.2
• Ammonia = 0.25 ppm
• Nitrites= 0 ppm
• Nitrates = 5 ppm

​

Update from 11/13/19. More trout are swimming! :)

Check out this video from 11/14/19!

11/4/19- 2 alevins and 1 egg removed. See picture posted below.

• ​Good eggs- 211
• Bad eggs- 55

11/5/19-​ N/A
​11/6/19- N/A
​11/7/19-
WATER QUALITY UPDATE

• pH = 7.6 
• High Range pH= 7.4
• Ammonia = 0.25 ppm
• Nitrites = 0 ppm
• Nitrates = 0.-5 ppm

​11/8/19-

10/28/19- I removed 21 eggs and alevins from the egg basket as well as a number of egg shells. Check out the picture below!

• Good eggs- 224
• Bad eggs- 42

10/29/19-  I removed 4 eggs and alevins from the egg basket as well as a number of egg shells.

• Good eggs- 220
• Bad eggs- 46​

10/30/19-  I removed 4 eggs and alevins from the egg basket as well as 3 egg shells. 

• Good eggs- 216
• Bad eggs- 50

WATER QUALITY UPDATE

• pH = 7.2
• Ammonia = 0.25 ppm 
• Nitrite = 0 ppm
• Nitrate = 0-5 ppm

10/31/19- I removed 2 alevins from the egg basket. Check out the pictures and videos below!

• Good eggs- 214
• Bad eggs- 52

​

Pictures below are from 10/28/19. The picture on the left is what I saw when I first opened the trout tank this morning. The picture on the right shows the egg shells, eggs, and alevins that were removed.

The picture below is from 10/30/19. You can see one of the trout did not hatch properly, and the other three are deformed such that their spines are too curved.

Below are pictures from 10/31/19.

​This is a compilation of three video clips showing a close-up of a trout alevin (sac-fry) breathing in a petri dish with trout tank water. Notice that there is a gap between the outside of the yellow yolk-sac and the outer membrane. This is a deformity in development. In normal development, the entire yolk sac and membrane will get smaller as the alevin consumes the yolk. However, when gas gets trapped and you see a gap, the alevin don't survive much longer.

Please note- the third video was taken using my phone as it would appear looking through the microscope. Due to automatic adjustments by the camera phone, this causes the image to look less clear.

Now that some of the eyed eggs have hatched, they are in the alevin or sac-fry stage. At this stage, the trout stay at the bottom of the egg basket and consume their yolk sac for nutrition. They are still too dense to swim to the surface, but when the water is disrupted, watch them wiggle!

Here is the YouTube link: ​https://youtu.be/h2MYt-GLuLk
Make sure you are viewing this in HD! Click on settings on the YouTube clip and click 1080p for the full resolution video.

Trout Club helped find the water quality of the trout tank.

• pH = 7.2
• Ammonia =  0.25 ppm
• Nitrites = 0 ppm
• Nitrates = 0 ppm
• GH = 107.4 ppm
• KH= 53.7 ppm

Week 1: Beginning 10/21/19

10/22/19- I removed one dead egg.

• Good eggs: 250
• Bad eggs: 16

10/23/19-  I removed two dead eggs.

• Good eggs: 248
• Bad eggs: 18

​10/24/19- N/A
​10/25/19- I removed three dead trout. This week, at least 15 egg shells have been removed. So many trout have hatched already!

• Good eggs: 245
• Bad eggs: 21

Below is a picture of the egg shells removed from the Trout Tank, as well as three alevins that were removed from the basket for suspicion of being dead. After looking in the microscope and not finding a heart beating, it was confirmed :(

During fourth period today, students in the Trout Team Club helped Mrs. Daniel to separate the good eggs from the bad (dead) eggs. You can tell an egg is dead because it looks whitish in color, rather than a translucent orange color with the eyes visible. 

Interestingly enough, it looked like a few eggs already hatched. Stay tuned!

TROUT COUNT- 10/21/19 Day 1

• Good eggs: 251
  
• Bad eggs: 15
• Total eggs received: 266